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Enrichment of Adult Mouse Dorsal Root Ganglia Neuron Cultures by Immunopanning
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1Neuroscience and Mental Health Institute,University of Alberta, 2Department of Medicine, Division of Neurology,University of Alberta, 3Department of Medical Microbiology and Immunology,University of Alberta, 4Department of Pharmacology,University of Alberta, 5Department of Anesthesiology and Pain Medicine,University of Alberta
Capítulos
- 00:04Introduction
- 00:43Dissection, Trituration, and Immunopanning
- 07:54Results: DRG Culture Neuronal Enrichment by Immunopanning
- 08:31Conclusion
This paper describes an immunopanning protocol for adult mouse dorsal root ganglia. By adhering antibodies to culture plates, we can negatively select and remove non-neuronal cells. We show that the cultures are enriched for neurons using this protocol, allowing for an in-depth study of neuronal responses to manipulation.
