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流动蚯蚓法在 秀丽隐 杆线虫肠道微生物组定量分析中的应用
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Biologia
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JoVE Journal Biologia
Application of Flow Vermimetry for Quantification and Analysis of the Caenorhabditis elegans Gut Microbiome
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流动蚯蚓法在 秀丽隐 杆线虫肠道微生物组定量分析中的应用

DOI:
10.3791/64605-v

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08:38 min

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March 31, 2023

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, , , ,
1Alkek Center for Metagenomics and Microbiome Research and Department of Molecular Virology and Microbiology,Baylor College of Medicine, 2Development, Disease Models and Therapeutics Program, Graduate School of Biomedical Sciences,Baylor College of Medicine

Capítulos

  • 00:05Introduction
  • 00:39Collecting Worm Population for Gut Microbiome Analyses
  • 02:08Setting Up the Large Particle Sorter and Autosampler
  • 03:43Sorting of Caenorhabditis elegans by Gut Microbiome Features
  • 06:05Results: Profiling Gut Microbiome Colonization
  • 07:50Conclusion

Summary

Tadução automática

Tadução automática

秀丽隐杆线虫 是一种强大的模型，用于研究驱动宿主-微生物组相互作用的分子决定因素。我们提出了一个高通量管道，分析肠道微生物组定植的单一动物水平以及 秀丽隐杆 线虫生理学的关键方面。

Tags

Flow CytometryGut MicrobiomeCaenorhabditis ElegansFluorescenceHigh-throughputReal-time MonitoringPhysiologyLevamisoleBleachParalysisWashAutosamplerLasersChannelsFlow Cytometer

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