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孔雀石绿测定法用于发现热休克蛋白 90 抑制剂

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors

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孔雀石绿测定法用于发现热休克蛋白 90 抑制剂

DOI:

10.3791/64693-v

•

07:57 min

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January 20, 2023

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Sayan Dutta Gupta, Dae-Geun Song, SueJung Lee, Jae Wook Lee, Jin-Soo Park, Chrisostomos Prodromou, Cheol-Ho Pan

¹Gangneung Institute of Natural Products,Korea Institute of Science and Technology (KIST), ²School of Life Sciences,University of Sussex

Capítulos

00:04Introduction
00:54Lab-Scale Malachite Green Assay
04:30High-Throughput Screening of Hsp90 Inhibitors by the Malachite Green Assay: Assay Design and Addition Orde
06:03Results: Reactions of Malachite Green Reagent, Standard Plot for Standard Phosphate Concentration, and IC₅₀ Calculation for Geldanamycin Against Yeast Hsp90
07:26Conclusion

Summary

Tadução automática

孔雀石绿测定方案是一种简单且经济高效的方法，用于发现热休克蛋白 90 （Hsp90）抑制因子以及其他针对 ATP 依赖性酶的抑制剂化合物。

Tags

Keywords: Malachite Green Assay Hsp90 ATPase Activity Phosphate Detection ATP Hydrolysis Inhibitors High-throughput Screening Geldanamycin Serial Dilution Blank Positive Control Absorbance

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