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Bioengineering

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JoVE Journal Bioengineering
Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells
 

Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells

Article DOI: 10.3791/64715-v • 09:46 min • January 20th, 2023
January 20th, 2023 •
1,2, 1,2,3,4
1School of Biomedical Engineering, University of British Columbia, 2Life Sciences Institute, University of British Columbia, 3Department of Medical Genetics, University of British Columbia, 4Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA)

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This protocol describes the generation of self-organizing blood vessels from human pluripotent and induced pluripotent stem cells. These blood vessel networks exhibit an extensive and connected endothelial network surrounded by pericytes, smooth muscle actin, and a continuous basement membrane.

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Human Blood Vessel Organoids Pluripotent Stem Cells Vasculogenesis Angiogenesis Vascular Disease Vascular Pathologies Reproducibility High Throughput Stem Cell Lines Diabetic Patient Vasculature Drug Treatment Avenues Stem Cell Population Aggregates Blood Vessel Organoid Generation HPSCs Cell Dissociation Reagent Aggregation Medium
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