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利用荧光原位杂交探索卵巢细胞中的X染色体畸变

JoVE Journal
Genética
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JoVE Journal Genética

Exploring X Chromosomal Aberrations in Ovarian Cells by Using Fluorescence In Situ Hybridization

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利用荧光原位杂交探索卵巢细胞中的X染色体畸变

DOI:

10.3791/64734-v

•

11:08 min

•

April 07, 2023

•

Sapthami Nadesapillai, Janielle van der Velden, Didi Braat, Kathrin Fleischer³, Ronald Peek

¹Department of Obstetrics and Gynecology,Radboudumc, ²Department of Pediatrics,Radboudumc, Amalia Children’s Hospital, ³Department of Reproductive Medicine,Nij Geertgen Center for Fertility

Capítulos

00:05Introduction
00:52Fluorescence In Situ Hybridization (FISH) on Isolated Individual Ovarian Cortex Cells
05:55FISH on Paraffin Sections of Grafted Ovarian Cortex Tissue
08:20Results: Determining X Chromosomal Content of Ovarian Cells in Non-Grafted and Grafted Ovarian Cortex Tissue
10:23Conclusion

Summary

Tadução automática

本文提出了两种基于荧光原位杂交的方法，以确定X染色体畸变女性非移植和移植卵巢皮层组织中卵巢细胞的X染色体含量。

Tags

Keywords: X Chromosomal Aberrations Ovarian Cells Fluorescence In Situ Hybridization (FISH)Stromal Cells Follicles Trypsin/EDTA/glucose Potassium Chloride DPBS Methanol-acetic Acid Fixation

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