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从人眼高效、一致地生成视网膜色素上皮/脉络膜平面安装，用于组织学分析

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis

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从人眼高效、一致地生成视网膜色素上皮/脉络膜平面安装，用于组织学分析

DOI:

10.3791/64761-v

•

07:59 min

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October 28, 2022

•

Davide Ortolan, Andrei Volkov, Arvydas Maminishkis, Ruchi Sharma, Kapil Bharti

¹Ocular and Stem Cell Translational Research Section, National Eye Institute,National Institutes of Health (NIH)

Capítulos

00:04Introduction
00:46Silicone Mold Preparation
01:21RPE Dissection
03:42Staining
04:45ReShAPE Analysis
06:17Results: Complete Morphometric Analysis of Entire Human RPE Monolayer
07:21Conclusion

Summary

Tadução automática

我们描述了一种有效地将视网膜色素上皮（RPE）与人眼视网膜分离并生成整个RPE /脉络膜平板安装以用于RPE的组织学和形态学分析的方法。

Tags

Keywords: Retinal Pigment Epithelium RPE Choroid Flatmount Histological Analysis Retinal Degeneration Dissection Technique REShAPE Software D-Mannitol DPBS PFA Macular Region Ciliary Margin

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