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Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain
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Biologia
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JoVE Journal Biologia
Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

DOI:
10.3791/64777-v

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07:50 min

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March 17, 2023

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, , , , , , ,
1Department of Agronomy, Center for Plant Biology,Purdue University, 2Department of Biology,Purdue University, 3Department of Horticulture, Faculty of Agricultural Sciences,University of the Punjab, 4Department of Botany and Plant Pathology, Center for Plant Biology,Purdue University

Capítulos

  • 00:04Introduction
  • 00:32Plant Material Propagation for Transformation
  • 02:05Agrobacterium tumefaciens Preparation
  • 03:34Transformation of Plantago Roots
  • 04:55Selection and Whole Plant Regeneration
  • 05:38Soil Transfer
  • 06:09Results: Agrobacterium tumefaciens-Mediated Genetic Transformation
  • 07:25Conclusion

Summary

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Tadução automática

Because of its versatile application as a model species in various fields of study, there is a need for a genetic transformation toolkit in narrowleaf plantain (Plantago lanceolata). Here, using Agrobacterium tumefaciens-mediated transformation, a protocol is presented that results in stable transgenic lines with a transformation efficiency of 20%.

Tags

Agrobacterium TumefaciensGenetic TransformationNarrowleaf PlantainPlantago SpeciesPlant Vascular BiologyStress PhysiologyExplantsPlantago LanceolataEthanolSodium HypochloriteMS MediumPetri DishGerminationAgrobacteriumPlasmidLB PlateSelection AgentLiquid LB

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