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Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain
 

Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

Article DOI: 10.3791/64777-v • 07:50 min • March 17th, 2023
March 17th, 2023 •
1, 1, 1, 2, 1, 3, 4, 1
1Department of Agronomy, Center for Plant Biology, Purdue University, 2Department of Biology, Purdue University, 3Department of Horticulture, Faculty of Agricultural Sciences, University of the Punjab, 4Department of Botany and Plant Pathology, Center for Plant Biology, Purdue University

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Because of its versatile application as a model species in various fields of study, there is a need for a genetic transformation toolkit in narrowleaf plantain (Plantago lanceolata). Here, using Agrobacterium tumefaciens-mediated transformation, a protocol is presented that results in stable transgenic lines with a transformation efficiency of 20%.

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Keywords: Agrobacterium Tumefaciens Genetic Transformation Narrowleaf Plantain Plantago Species Plant Vascular Biology Stress Physiology Explants Plantago Lanceolata Ethanol Sodium Hypochlorite MS Medium Petri Dish Germination Agrobacterium Plasmid LB Plate Selection Agent Liquid LB
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