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离体 聚乙烯醇基培养物中小鼠造血干细胞的扩增和遗传操作
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Biologia
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JoVE Journal Biologia
Ex Vivo Expansion and Genetic Manipulation of Mouse Hematopoietic Stem Cells in Polyvinyl Alcohol-Based Cultures
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离体 聚乙烯醇基培养物中小鼠造血干细胞的扩增和遗传操作

DOI:
10.3791/64791-v

•

07:55 min

•

February 10, 2023

•

, ,
1MRC Weatherall Institute of Molecular Medicine,University of Oxford

Capítulos

  • 00:05Introduction
  • 01:05Bone Marrow Extraction and c-Kit&#43 Enrichment of Hematopoietic Stem Cell &#40HSC&#41
  • 03:03Initiating Cell Cultures with c-Kit-Enriched Hematopoietic Stem and Progenitor Cells &#40HSPCs&#41
  • 03:45Performing Media Changes
  • 04:19Electroporating Cultured HSPCs
  • 05:24Transducing Cultured HSPCs with Lentiviral Vector
  • 06:17Results: Fluorescence-Activated Cell Sorting &#40FACS&#41 of the c-Kit-Enriched HSCs
  • 07:22Conclusion

Summary

Tadução automática

Tadução automática

这里介绍的是使用 离体 聚乙烯醇基扩增启动、维持和分析小鼠造血干细胞培养物的方案，以及通过慢病毒转导和电穿孔进行遗传操作的方法。

Tags

Hematopoietic Stem CellsEx Vivo ExpansionGenetic ManipulationPolyvinyl AlcoholMouseBone MarrowC-KitMagnetic Column SeparationCell Culture

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