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Journal / Immunology and Infection / Gene Editing of Primary Rhesus Macaque B Cells
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Immunology and Infection

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JoVE Journal Immunology and Infection
Gene Editing of Primary Rhesus Macaque B Cells
 

Gene Editing of Primary Rhesus Macaque B Cells

Article DOI: 10.3791/64858-v • 09:53 min • February 10th, 2023
February 10th, 2023 •
1, 2, 2, 3,5, 1, 1,6, 1, 2, 1,4
1Laboratory of Molecular Immunology, The Rockefeller University, 2Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 3Laboratory of Retrovirology, The Rockefeller University, 4Howard Hughes Medical Institute, The Rockefeller University, 5Laboratory of Applied Virology and Precision Medicine, King Abdullah University of Science and Technology (KAUST), 6Vaccine and Immunotherapy Center, Wistar Institute

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Resumo
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We present a method for culturing and gene editing primary rhesus macaque B cells using CRISPR/Cas9 and recombinant adeno-associated virus serotype 6 for the study of B cell therapies.

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Gene Editing Primary Rhesus Macaque B Cells Method Efficient Therapeutic Purposes Preclinical Research Cell Types GRNA Cutting Efficiency Recombinant AAV6 B Cell Culture Media B Cell Stimulants Thawing Media Splenocytes PBMCs Centrifuge Washing Medium Cell Suspension Trypan Blue Cell Concentration
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