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A GMP-Compliant Procedure for the Generation of Gene-Modified T cells
JoVE Journal
Bioengenharia
Author Produced
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JoVE Journal Bioengenharia
A GMP-Compliant Procedure for the Generation of Gene-Modified T cells
DOI:

06:47 min

October 06, 2023

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Capítulos

  • 00:00Introduction
  • 00:38Day 0: Peripheral Blood Mononuclear Cells (PBMC) Isolation and T Cell Activation
  • 01:42Day 3: Lentiviral T Cell Transduction
  • 03:04Day 4: Virus Removal and T Cell Expansion
  • 03:44Day 14: Cryopreservation
  • 04:39Transduction Efficiency-Flow Cytometry Staining (Day 14)
  • 05:35Results: Assessment of the Transduced Human T Cells
  • 06:29Conclusion

Summary

Tadução automática

This protocol outlines the process of transducing primary human T cells with a gene of interest, ensuring compatibility with Good Manufacturing Practice (GMP) standards.

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