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Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
 

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue

Article DOI: 10.3791/65153-v • 04:41 min • July 28th, 2023
July 28th, 2023 •
1, 1, 1, 1, 1, 1
1Dermatology Hospital, Southern Medical University

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Resumo
Automatic Translation

This study describes an optimized protocol for establishing primary fibroblasts from keloid tissues that can effectively and steadily provide pure and viable fibroblasts.

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Isolation Culture Primary Dermal Fibroblasts Human Keloid Tissue Keloid Research Sterile Tweezers Sterile Centrifuge Tube PBS Supplemented With 1% PSA Six-well Plate Surgical Scissors Dermis Tissue Pieces Petri Dishes Incubator DMEM Supplemented With 10% FBS And 1% PSA Complete Culture Media
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