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破骨细胞与人诱导多能干细胞的分化和表征
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells
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破骨细胞与人诱导多能干细胞的分化和表征

DOI:
10.3791/66527-v

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10:52 min

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March 22, 2024

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, , , ,
1Department of Bioengineering, Department of Medicine,University of Washington, 2Department of Orthopedics and Trauma Surgery, Medical Faculty Mannheim,Heidelberg University

Capítulos

  • 00:03Introduction
  • 01:16Passaging iPSCs
  • 03:32Embryoid Body Induction
  • 06:10Hematopoietic Differentiation
  • 08:31M-CSF Maturation and Osteoclast Differentiation
  • 09:25Representative Results
  • 10:19Conclusion

Summary

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该方案介绍了人破骨细胞与诱导多能干细胞（iPSCs）的分化，并描述了破骨细胞和破骨细胞前体的表征方法。

Tags

OsteoclastsHuman Induced Pluripotent Stem Cells (iPSCs)DifferentiationCharacterizationPassagingCD34+ Peripheral Blood Mononuclear CellsBone Disease ResearchPesquisa do CâncerEndoprosthesis ResearchExpandabilityDMEM/F-12DispaseHEPESCell CultureCell Aggregates

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