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Shrinky-Dink Hanging Drops: A Simple Way to Form and Culture Embryoid Bodies
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Biologia
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JoVE Journal Biologia
Shrinky-Dink Hanging Drops: A Simple Way to Form and Culture Embryoid Bodies

Shrinky-Dink Hanging Drops: A Simple Way to Form and Culture Embryoid Bodies

DOI:
10.3791/692-v

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07:23 min

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March 05, 2008

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, , , , , ,
1School of Engineering,University of California Merced – UC Merced

Capítulos

  • 00:00Título
  • 01:31Making Shrinky-Dink Mold
  • 02:57Making PDMS Microwells
  • 05:05Trapping Cells in Microwells
  • 06:43Conclusion

Summary

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Tadução automática

We show a simple and rapid method to load pre-defined numbers of cells into microfabricated wells and maintain them for embryoid body development.

Tags

Shrinky-Dink Hanging DropsSimple WayForm And CultureEmbryoid BodiesEmbryonic Stem CellsHanging Drop MethodPipettingAggregatesStem Cell DifferentiationMicrofabricated WellsRed Fluorescent Mouse Cell Line

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