/

/

从胎盘中的造血干细胞的分离和分析

JoVE Journal
Biologia

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Biologia

Isolation and Analysis of Hematopoietic Stem Cells from the Placenta

Please note that all translations are automatically generated. Click here for the English version.

从胎盘中的造血干细胞的分离和分析

DOI:

•

14:42 min

•

June 24, 2008

•

Christos Gekas, Katrin E. Rhodes, Hanna K. A. Mikkola

¹Jonsson Comprehensive Cancer Center,University of California, Los Angeles

Capítulos

00:05Título
01:45Embryo removal and placental dissection
06:01Single-cell suspension of placenta
10:22Embedding the fixed tissue
12:25Expected tissue section results
13:23Conclusion

Summary

Tadução automática

我们已经确定了在开发过程中作为一个主要的造血器官，胎盘。我们发现，造血干细胞（HSCs）都产生和扩大，在胎盘中的独特的微环境龛。在这里，我们描述了在小鼠胎盘中的造血干细胞的分离和可视化所需的实验技术。

Tags

Hematopoietic Stem Cells Placenta Isolation Analysis Self-renewal Blood Lineages Embryonic Development Pool Size Anatomical Origin Fetal Hematopoiesis Microenvironmental Niches Dissection Techniques Murine Placenta Developmental Stages HSC Pool Size Enzymatic Dissociation Mechanical Dissociation Flow Cytometry Functional Assays

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

分离和造血干细胞移植（造血干细胞）

从小鼠胚胎干细胞的造血干细胞的推导

小鼠卵黄囊和AGM早期造血干细胞的分离

从鼠眼视网膜干细胞的分离

慢病毒介导的击倒在体外红细胞

表型分析和分离小鼠造血干细胞和传承的承诺祖

诱导上皮和分析间质转化

从成年小鼠切牙牙源性上皮干细胞的分离和培养

小鼠胚胎的逆转录病毒感染干细胞衍生胚体细胞造血分化的分析

在EML造血前体细胞调节自我更新和分化的关键因素，通过RNA测序分析鉴定

Read Article