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In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging
 

In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging

Article DOI: 10.3791/827-v • 11:41 min • August 3rd, 2008
August 3rd, 2008 •
1, 1
1Division of Cardiovascular Medicine, Stanford University

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In this video, we are showing how to label human embryonic stem cells (hESC) with manganese chloride (MnCl2) which can enter cells via voltage-gated calcium channels when the cells are biologically active. Additionally, we show the use of MnCl2 as cellular MRI contrast agent to determine the in vitro viability of hESC.

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In Vitro Labeling Human Embryonic Stem Cells Magnetic Resonance Imaging MRI Iron-oxide Nanoparticles Transplanted Stem Cells Manganese Chloride Ca2+ Channels T1 Shortening Effect Cell Viability Myocardium In Vitro MRI Imaging
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