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研究与基于荧光素酶的记者基因检测膜生源
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Biologia
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JoVE Journal Biologia
Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay
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研究与基于荧光素酶的记者基因检测膜生源

DOI:
10.3791/920-v

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13:48 min

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September 07, 2008

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,
1Department of Molecular and Cell Biology,Harvard, 2Molecular and Metabolic Signalling Centre, Division of Basic Medical Sciences,St. George’s University of London

Capítulos

  • 00:00Título
  • 00:49Introduction
  • 01:21Preparing Transfections
  • 05:35Inducing Membrane Biosynthesis via Phagocytosis of Latex Beads
  • 08:07Performing the Dual-Glo Luciferase Assays
  • 11:28Data Analysis
  • 13:00Conclusion

Summary

Tadução automática

Tadução automática

在这里，我们描述基于荧光素酶报告基因的使用自Promega的双GloTM荧光素酶检测系统的方法研究细胞的吞噬功能，诱导基因表达的变化的过程。

Tags

Membrane BiogenesisLuciferase-based Reporter Gene AssayLipid Synthesis PathwaysExperimental SystemPhagocytosisLatex BeadsMembrane LipidsGene ExpressionDual-Glo Luciferase Assay System

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