JOVE JOURNAL

Biochemistry

Showing 157 - 168 in 731 videos.

DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density

Chelcie F. Heaney², Colin J. McArdle, Kimberly F. Raab-Graham²

¹Wake Forest Translational Alcohol Research Center,Wake Forest School of Medicine, ²Physiology and Pharmacology,Wake Forest School of Medicine

Journal (Biochemistry)

Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy

Jessica L. Henty-Ridilla

¹Department of Biochemistry & Molecular Biology, and Department of Neuroscience & Physiology,State University of New York (SUNY) Upstate Medical University

Journal (Biochemistry)

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

Liza-Anastasia DiCecco*^1,2, Samantha Berry*¹, G. M. Jonaid*^1,3, Maria J. Solares⁴, Liam Kaylor⁴, Jennifer L. Gray, Carol Bator, William J. Dearnaley, Michael Spilman, Madeline J. Dressel-Dukes, Kathryn Grandfield, Sarah M. McDonald Esstman, Deborah F. Kelly^5,6

¹Department of Biomedical Engineering,Pennsylvania State University, ²Department of Materials Science and Engineering,McMaster University, ³Bioinformatics and Genomics Graduate Program, Huck Institutes of the Life Sciences,Pennsylvania State University, ⁴Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences,Pennsylvania State University, ⁵Materials Research Institute,Pennsylvania State University, ⁶Huck Institutes of the Life Sciences,Pennsylvania State University, ⁷Applications team,Direct Electron, ⁸Application Scientist,Protochips, Inc., ⁹Department of Biology,Wake Forest University

Journal (Biochemistry)

Inner Mitochondrial Membrane Sensitivity to Na⁺ Reveals Partially Segmented Functional CoQ Pools

Pablo Hernansanz-Agustín, José Antonio Enríquez²

¹Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III CNIC, ²Centro de Investigaciones Biomédica en Red de Fragilidad y Envejecimiento, Saludable-CIBERFES

Journal (Biochemistry)

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

Satyaghosh Maurya, Vishwesh Haricharan Rai, Aditya Upasani, Saurabh Umrao², Diksha Parwana, Rahul Roy³

¹Department of Chemical Engineering,Indian Institute of Science, ²Holonyak Micro & Nanotechnology Lab,University of Illinois, Urbana-Champaign, ³Center for BioSystems Science and Engineering,Indian Institute of Science

Journal (Biochemistry)

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon

Sriram Aiyer, Timothy S. Strutzenberg, Marianne E. Bowman, Joseph P. Noel³, Dmitry Lyumkis^4,5

¹Laboratory of Genetics,The Salk Institute for Biological Studies, ²Jack H. Skirball Center for Chemical Biology and Proteomics,The Salk Institute for Biological Studies, ³Department of Chemistry and Biochemistry,University of California San Diego, ⁴Graduate School of Biological Sciences, Section of Molecular Biology,University of California San Diego, ⁵Department of Integrative Structural and Computational Biology,The Scripps Research Institute

Journal (Biochemistry)

The Peel-Blot Technique: A Cryo-EM Sample Preparation Method to Separate Single Layers From Multi-Layered or Concentrated Biological Samples

Matthew C. Johnson*^1,2, Arshay J. Grant*¹, Ingeborg Schmidt-Krey³

¹School of Biological Sciences,Georgia Institute of Technology, ²Department of Structural Biology,Genentech, ³School of Chemistry and Biochemistry,Georgia Institute of Technology

Journal (Biochemistry)

Self-Assembly of Microtubule Tactoids

Prashali Chauhan, Sumon Sahu², Niaz Goodbee, Sophia Martin, Hong Beom Lee, Ruell Branch, Jennifer M. Schwarz, Jennifer L. Ross

¹Physics Department,Syracuse University, ²Department of Molecular Biophysics and Biochemistry,Yale School of Medicine

Journal (Biochemistry)

Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

Gerard Castro-Linares, Jeffrey den Haan, Francois Iv, Carla Silva Martins, Aurélie Bertin, Manos Mavrakis, Gijsje H. Koenderink

¹Department of Bionanoscience, Kavli Institute of Nanoscience Delft,Delft University of Technology, ²Institut Fresnel, CNRS UMR7249,Aix Marseille Univ, Centrale Marseille, ³Institut Curie, Université PSL,Sorbonne Université

Journal (Biochemistry)

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

Lin Cheng*¹, Lantian Su*¹, Xiaowen Tian*¹, Fan Xia, Chang Zhao, Wei Yan, Zhenhua Shao

¹Division of Nephrology and Kidney Research Institute, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital,Sichuan University, ²Department of Neurosurgery, West China Hospital,Sichuan University

Journal (Biochemistry)

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

Elizabeth M. Bailey, Emanuel Salazar-Cavazos, Rachel M. Grattan, Michael J. Wester, David J. Schodt, Julian A. Rojo, Keith A. Lidke³, Diane S. Lidke³

¹Department of Pathology,University of New Mexico School of Medicine, ²Department of Physics and Astronomy,University of New Mexico, ³Comprehensive Cancer Center,University of New Mexico Health Sciences Center

Journal (Biochemistry)

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

Armand M. G. Jaminon*¹, Nikolas Rapp*¹, Asim C. Akbulut, Robert Dzhanaev, Chris P. Reutelingsperger, Willi Jahnen-Dechent, Leon J. Schurgers³

¹Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM),Maastricht University, ²Helmholtz Institute for Biomedical Engineering, Biointerface Group,RWTH Aachen University, ³Institute of Experimental Medicine and Systems Biology,RWTH Aachen University

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