JOVE JOURNAL

Biochemistry

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A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

David J. Samuels, Zhe Wang, Kyu Y. Rhee³, Shaun R. Brinsmade

¹Department of Biology,Georgetown University, ²Division of Infectious Diseases,Weill Cornell Medical College, ³Department of Medicine,Weill Cornell Medical College

Journal (Biochemistry)

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures

Andrew Baxter, Emmanuel Minet

¹Research and Development,British American Tobacco

Journal (Biochemistry)

A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation

Christopher J. A. Warner, Subrata Dutta, Alejandro R. Foley, Jevgenij A. Raskatov

¹Department of Chemistry,University of California, Santa Cruz

Journal (Biochemistry)

Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

Stamatios Liokatis

¹Department of Structural Biology,Leibniz Institute of Molecular Pharmacology (FMP-Berlin)

Journal (Biochemistry)

Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants

Hyelee Lee, Shih-Yuan Liu

¹Department of Chemistry,Boston College

Journal (Biochemistry)

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Eric D. Routh*¹, Steven D. Creacy*², Peter E. Beerbower, Steven A. Akman, James P. Vaughn, Philip J. Smaldino

¹Department of Cancer Biology,Wake Forest School of Medicine, ²YX Genomics, ³Department of Biology,Ball State University, ⁴Department of Hematology and Oncology,Roper St. Francis Hospital

Journal (Biochemistry)

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

Ramakrishnan B. Kumar, Lin Zhu, Hans Hebert², Caroline Jegerschöld²

¹Department of Biosciences and Nutrition,Karolinska Institutet, ²School of Technology and Health,KTH Royal Institute of Technology

Journal (Biochemistry)

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Robyn M. Johnston, Anne W. Sylvester, Michael J. Scanlon

¹Plant Biology Section, School of Integrative Plant Science,Cornell University, ²Department of Developmental Genetics,University of Wyoming

Journal (Biochemistry)

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Ibrahim B. Durowoju, Kamaljit S. Bhandal, Jian Hu, Bruce Carpick, Marina Kirkitadze

¹Analytical Research & Development,Sanofi Pasteur Limited

Journal (Biochemistry)

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

Lisa M. Meints, Anne W. Poston, Brent F. Piligian, Claire D. Olson, Katherine S. Badger, Peter J. Woodruff, Benjamin M. Swarts

¹Department of Chemistry and Biochemistry,Central Michigan University, ²Department of Chemistry,University of Southern Maine

Journal (Biochemistry)

Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System

Thilo Dörfler*¹, Tobias Eilert*¹, Carlheinz Röcker, Julia Nagy, Jens Michaelis

¹Institute of Biophysics,Ulm University

Journal (Biochemistry)

A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

Irena Roci², Hector Gallart-Ayala, Jeramie Watrous, Mohit Jain, Craig E. Wheelock, Roland Nilsson²

¹Department of Medicine, Unit of Computational Medicine,Karolinska Institutet, ²Center for Molecular Medicine,Karolinska Institutet, ³Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry 2,Karolinska Institutet, ⁴Department of Medicine,University of California San Diego

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