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Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
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Biochemistry
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JoVE Journal Biochemistry
Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

DOI:
10.3791/54485-v

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09:27 min

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February 17, 2017

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, , , , , ,
1Department of Chemistry and Biochemistry,Central Michigan University, 2Department of Chemistry,University of Southern Maine

Chapters

  • 00:05Title
  • 01:14Expression and Purification of Trehalose Synthase Thermoproteus tenax (TreT)
  • 04:04Synthesis and Purification of a Trehalose Analogue — Exemplified with the Synthesis of 6-Azido-trehalose (6-TreAz)
  • 06:59Results: One-step Synthesis of Trehalose Analogues Using TreT
  • 08:14Conclusion

Summary

Automatic Translation

Automatic Translation

Trehalose analogues are emerging as important molecules for bio(techno)logical and biomedical applications. We describe an optimized protocol for enzymatically synthesizing and purifying trehalose analogues that is simple, efficient, fast, and environmentally friendly. Its application to the rapid production and administration of a probe for the detection of mycobacteria is demonstrated.

Tags

Keywords: Trehalose AnaloguesEnzymatic SynthesisAll-aqueous PurificationTreT ExpressionE. ColiTuberculosis DetectionBiopreservationNoncaloric Sweeteners

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