All Protocols and Video Articles for scientific research | JoVE

Showing 385 - 396 of 477 videos for "Reconstitution"

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Journal (Biology)

Rapid Generation of Amyloid from Native Proteins In vitro

Stephanie M Dorta-Estremera, Jingjing Li, Wei Cao

¹Department of Immunology,The University of Texas MD Anderson Cancer Center

Journal (Biology)

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

Ido Karady, Anna Frumkin, Shiran Dror, Netta Shemesh, Nadav Shai, Anat Ben-Zvi

¹Department of Life Sciences, National Institute for Biotechnology in the Negev,Ben-Gurion University of the Negev

Journal (Immunology and Infection)

Rescue of Recombinant Newcastle Disease Virus from cDNA

Juan Ayllon², Adolfo García-Sastre^2,3, Luis Martínez-Sobrido

¹Department of Microbiology,Icahn School of Medicine at Mount Sinai, ²Global Health and Emerging Pathogens Institute,Icahn School of Medicine at Mount Sinai, ³Department of Medicine,Icahn School of Medicine at Mount Sinai, ⁴Department of Microbiology and Immunology, School of Medicine and Dentistry,University of Rochester

Journal (Biology)

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Zachary Z. Sun*¹, Clarmyra A. Hayes*², Jonghyeon Shin, Filippo Caschera, Richard M. Murray, Vincent Noireaux

¹Department of Biology,California Institute of Technology, ²Department of Bioengineering,California Institute of Technology, ³Synthetic Biology Center, Department of Bioengineering,Massachusetts Institute of Technology, ⁴School of Physics and Astronomy,University of Minnesota

Journal (Immunology and Infection)

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

Ziming Cheng, Ting Zhou², Azhar Merchant, Thomas J. Prihoda, Brian L. Wickes⁵, Guogang Xu, Christi A. Walter⁵, Vivienne I. Rebel^2,5

¹Greehey Children's Cancer Research Institute,UT Health Science Center at San Antonio, ²Department of Cellular and Structural Biology,UT Health Science Center at San Antonio, ³Department of Pathology,UT Health Science Center at San Antonio, ⁴Department of Microbiology,UT Health Science Center at San Antonio, ⁵Cancer Therapy and Research Center,UT Health Science Center at San Antonio

Journal (Immunology and Infection)

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

Lisa M. Weatherly*^1,2, Rachel H. Kennedy*^1,2, Juyoung Shim, Julie A. Gosse²

¹Department of Molecular and Biomedical Sciences,University of Maine, Orono, ²Graduate School of Biomedical Sciences and Engineering,University of Maine, Orono

Journal (Bioengineering)

Production of Large Numbers of Size-controlled Tumor Spheroids Using Microwell Plates

Golsa Razian, Yang Yu, Mark Ungrin

¹Department of Comparative Biology & Experimental Medicine, Faculty of Veterinary Medicine,University of Calgary

Journal (Biology)

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation

Jessica M Bryant², Mirella L Meyer-Ficca, Vanessa M Dang, Shelley L Berger^4,5, Ralph G Meyer

¹Department of Cell and Developmental Biology,University of Pennsylvania, ²Biomedical Graduate Studies,University of Pennsylvania, ³Department of Animal Biology and Center for Animal Transgenesis and Germ Cell Research,University of Pennsylvania, ⁴Department of Genetics,University of Pennsylvania, ⁵Department of Biology,University of Pennsylvania

Journal (Biology)

Viability Assays for Cells in Culture

Jessica M. Posimo, Ajay S. Unnithan, Amanda M. Gleixner, Hailey J. Choi, Yiran Jiang, Sree H. Pulugulla, Rehana K. Leak

¹Division of Pharmaceutical Sciences, Mylan School of Pharmacy,Duquesne University

Journal (Immunology and Infection)

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Michael Müller, Jana Becher, Matthias Schnabelrauch, Marcy Zenobi-Wong

¹Department of Health Science & Technology,Cartilage Engineering & Regeneration, ²Biomaterials Department,Innovent e.V.

Journal (Medicine)

The In ovo CAM-assay as a Xenograft Model for Sarcoma

Gwen M.L. Sys, Lore Lapeire, Nikita Stevens, Herman Favoreel, Ramses Forsyth, Marc Bracke, Olivier De Wever

¹Department of Orthopaedic Surgery and Traumatology,Ghent University Hospital, ²Department of Radiation Oncology and Experimental Cancer Research,Ghent University, ³Department of Virology, Parasitology, and Immunology,Ghent University, ⁴Pathlicon

Journal (Biology)

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

James Smadbeck, Meghan B. Peterson, George A. Khoury, Martin S. Taylor, Christodoulos A. Floudas

¹Department of Chemical and Biological Engineering,Princeton University

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