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Immunology and Infection
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Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
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Immunology and Infection
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JoVE Journal Immunology and Infection
Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

DOI:
10.3791/50632-v

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10:49 min

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July 10, 2013

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, , ,
1Department of Health Science & Technology,Cartilage Engineering & Regeneration, 2Biomaterials Department,Innovent e.V.

Chapters

  • 00:05Title
  • 01:36Preparation of the Poloxamer 407 Solution
  • 02:58Preparation of the 3D Printer
  • 04:24Optimization of the Printing Parameters
  • 06:42Printing and Elution of the Reverse Mold and Filling of the Voids
  • 09:38Results: Patterned Two-component Hydrogel Printing
  • 10:21Conclusion

Summary

Automatic Translation

Automatic Translation

A bioprinter was used to create patterned hydrogels based on a sacrificial mold. The poloxamer mold was backfilled with a second hydrogel and then eluted, leaving voids which were filled with a third hydrogel. This method uses fast elution and good printability of poloxamer to generate complex architectures from biopolymers.

Tags

Keywords: BioprintingThermoresponsiveReverse MoldTwo-component Hydrogel3D Cell CulturePoloxamerAgaroseAlginate MethacrylateFITC-fibrinogenExtrusion BioprintingSacrificial MoldVascular NetworkNerve Guide Conduit

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