走在大鼠作为定性分析方法使用Rotorod
Summary
rotorod测试是用来评估半侧帕金森模拟大鼠的行走运动的电机状态。
Abstract
rotorod测试，在一个转鼓上行走的动物，被广泛用于评估在啮齿类实验动物的运动状态。性能测量的持续时间，动物保持鼓，转鼓转速的功能。在这里，我们报告的任务，提供了丰富的步行运动的定性方面的信息来源。因为运动是在一个固定的位置进行的，他们可以很容易地用高速视频记录方法研究。目前的研究进行了检查rotorod潜力揭示半帕金森模拟到右侧黑质纹状体束注射6 – 羟多巴胺（6 – OHDA）制作大鼠，步行运动的质的变化，消耗黑质纹状体多巴胺（DA ）。翌日，手术开始，然后定期在未来的两个月中，老鼠是从正面，横向，和后部的看法拍摄，因为他们走进rotorod。帧帧回放视频记录的行为进行了分析。加强行为评定量表表明，半帕金森大鼠长期在他们的姿势和使用的肢体对侧的DA消耗受损。不仅显示姿势和运动异常，对侧肢体，他们参加发起和持续推进比同侧肢体。这些研究结果不仅揭示继发单方面的DA消耗新的赤字，但也表明，rotorod可以提供一个强大的工具，对运动的定性分析。
Protocol
Rotorod步行仪 rotorod是一个旋转的圆柱体，直径4厘米，高出地面35厘米，由透明有机玻璃封闭固定。 rotorod缸覆盖质感的橡胶涂层，这有利于牵引。仪器的地板所用的泡沫层，以防止伤害动物下跌时。小型电动马达提供的电源打开通过橡胶带rotorod。 rotorod是轮换一次，每5秒录像和运动学一个高速的数码摄像机，用于拍摄动物，使用了1000秒?…
Discussion
本研究的目的是研究是否可以用于定性检查的姿势，前肢加强和后肢踩着在中枢神经系统损伤大鼠rotorod灭鼠运动技能测试。单方面DA枯竭和对照组大鼠进行了分析，录制的视频前，外侧和后侧的意见。一个姿势和前肢动作的评定量表表明，加强运动，手术后长期受损。肢体运动的研究表明，而DA耗尽的老鼠可以使用支持病变对侧肢体，他们收到的转移重量极少使用。这项研究的结果表明rotorod任务，除了提供定量?…
Acknowledgements
由加拿大卫生研究院，加拿大中风网络，和阿尔伯塔省文物基金会的医学研究机构的拨款支持。
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Tags
RotorodQualitative AnalysisWalking In RatsHigh-speed Video AnalysisRepetitive MovementsFoot Stepping MovementsHemi-Parkinson Rats6-hydroxydopamine (6-OHDA)Nigrostriatal BundleDopamine DepletionVideo Analysis IllustrationFrontal ViewLateral ViewPosterior ViewStepping BehaviorPosture ImpairmentLimb Use ImpairmentContralateral LimbsIpsilateral LimbsDeficits Secondary To Unilateral DA-depletion
Cite This Article
Whishaw, I. Q., Li, K., Whishaw, P. A., Gorny, B., Metz, G. A. Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat. J. Vis. Exp. (22), e1030, doi:10.3791/1030 (2008).