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JoVE Journal > Developmental Biology
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Developmental Biology
使用FACS分选的小鼠卫星细胞的核酸酶分析在靶标下进行切割和释放
Published: July 07, 2023
doi:
10.3791/200376
Kamar Ghaibour*
1
,
Joe Rizk*
1
,
Claudine Ebel
,
Tao Ye
,
Muriel Philipps
,
Valérie Schreiber
,
Daniel Metzger
,
Delphine Duteil
1
CNRS, Inserm, IGBMC UMR 7104- UMR-S 1258
,
Université de Strasbourg
Tags
Keywords: Satellite Cells
FACS
Nuclear Extraction
Concanavalin A
Magnetic Beads
Binding Buffer
Wash Buffer
Micrococcal Nuclease
Calcium Chloride
Phenol:chloroform:isoamyl Alcohol
Proteinase K
SDS
check_url/200376?article_type=t
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Cleavage Under Targets and Release Using Nuclease Analysis of FACS-Sorted Mouse Satellite Cells.
J. Vis. Exp.
(Pending Publication), e200376, doi:10.3791/200376 (2023).
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