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JoVE Journal > Bioengineering
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Bioengineering
التوسع ثنائي المستوى للخلايا الجذعية الوسيطة البشرية من مفاعلات حيوية سعة 5 لتر إلى 15 لترا وصياغة آلية للخلايا من مفاعل حيوي سعة 15 لترا
Published: July 07, 2023
doi:
10.3791/200420
Yuyang Chen*
1
,
Huanye Xu*
2
,
Yuanyuan Zhang*
2
,
Leilei Guo
,
Miaomiao Lan
,
Yanping Yang
,
Wei Liu
,
Xiaojun Yan
,
Yanan Du
1
Department of Biomedical Engineering, School of Medicine
,
Tsinghua University
,
2
Beijing CytoNiche Biotechnology Co. Ltd.
Tags
Keywords: Mesenchymal Stem Cells
Bioreactor
Cell Expansion
Automated Cell Formulation
Cell Harvest
Cell Processing
Microcaririer
Serum-free Medium
Cell Inoculation
Medium Supplement
Medium Exchange
Cell Sampling
Cell Harvest
Cell Suspension
Cell Washing
Cell Culture
check_url/200420?article_type=t
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Two-Tiered Expansion of Human Mesenchymal Stem Cells from 5-Liter to 15-Liter Bioreactors and Automated Cell Formulation from a 15-Liter Bioreactor.
J. Vis. Exp.
(Pending Publication), e200420, doi:10.3791/200420 (2023).
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