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Biology
Quantificação Fluorométrica e Análise Citométrica de Armadilhas Extracelulares de Neutrófilos (NETs) de Rato
Published: April 26, 2024
doi:
10.3791/200812
Xiangfeng Gong*
1
,
Yutao Sun*
1
,
Xiaoxin Zhang
3
,
Zhenghua Xiao
4
,
Li He
4
,
Chaoyi Qin
4
1
Department of Cardiovascular Surgery, West China Hospital
,
Sichuan University
,
2
Department of Integrated Traditional Chinese and Western Medicine, Sichuan Provincial Pancreatitis Centre, West China Hospital
,
Sichuan University
,
3
West China-Liverpool Biomedical Research Centre, West China Hospital
,
Sichuan University
,
4
Cardiovascular Surgery Research Laboratory, West China Hospital
,
Sichuan University
Tags
Keywords: Fluorometric Quantification
Cytometric Analysis
Rat Neutrophil Extracellular Traps (NETs)
Tris-EDTA Buffer
DNA Standards
Assay Reagent
Fluorescence Microplate Reader
Nucleus Stain
Cell-free DNA Stain
Cytometer
SYTOX Green
HEXT Channel
PMA Stimulation
Cell-free DNA Content
check_url/200812?article_type=t
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Fluorometric Quantification and Cytometric Analysis of Rat Neutrophil Extracellular Traps (NETs).
J. Vis. Exp.
(Pending Publication), e200812, doi:10.3791/200812 (2024).
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