In this video, we demonstrate the procedure for administering intra-prostatic injection of prostate cancer cells into a mouse model. Orthotopic murine models can be used to study the disease origin and response to treatment therapies in a non-invasive and clinically relevant environment.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Pre-operative Mouse Preparation NOTE: House mice in the university animal facility for at least one week before surgery to allow for adequate adaptation and minimize animal stress. Steps 1.1-1.6 are performed by the surgeon’s assistant. All subsequent surgical steps a…
Representative Results
Figure 1: Anterior prostate lobe, draining lymph nodes, and representative technique for intra-prostatic cell injections. Images of the (A) right anterior prostate lobe (black, *), attached right seminal vesicle (white), right testicle and fat pad (green), and bladder (yellow), (B) bilateral prostate-draining para-aortic lymph nodes (orange), (C)…
Disclosures
The authors have nothing to disclose.
Materials
Myc-CaP cell line
ATCC
CRL-3255
Verify as mycoplasma-free before use
Micro-dissecting scissors
Roboz
RS-5910
Graege forceps
Roboz
RS-5135
Graefe tissue forceps
Roboz
RS-5150
Olsen-Hegar needle holder with
suture cutters
FST
12002-12
50 µL Syringe 705 RN SYR
Hamilton
7637-01
Sterilize by ethylene oxide gas
28-gauge Needles Small Hub RN H
Hamilton
7803-02
Point style 4, Angle 45°, Length 0.75 in. Sterilized by ethylene oxide gas
Intra-prostatic Injection of Cancer Cells: A Technique to Deliver Cancer Cells for Establishing Orthotopic Prostate Cancer Mouse Model. J. Vis. Exp. (Pending Publication), e20398, doi: (2023).