Periodic Acid Schiff or PAS Staining: A Method to Assess the Structural Deformities of Glomerulus
Published: April 30, 2023
Abstract
Source: Stevens, M., Oltean, S. Assessment of Kidney Function in Mouse Models of Glomerular Disease. J. Vis. Exp. (2018)
In this video, we describe the protocol for performing Periodic Acid Schiff (PAS) stain to assess the structural anomalies in the kidney glomerulus. The PAS staining method enables appropriate evaluation of the glomerulus pathophysiology.
Protocol
1. Periodic Acid Schiff (PAS) Stain NOTE: The PAS stain will highlight the basement membranes of glomerular capillary loops and the tubular epithelium. It enables detailed visualization of the glomerular cells, mesangial matrix and potential expansion, and potential changes of the GBM (i.e., thickening and irregularities). Section the paraffin-embedded, PFA-fixed kidney cortex using a microtome at 5 µm thickness onto poly-L-lysine coated slid…
Disclosures
The authors have nothing to disclose.
Materials
| Periodic Acid-Schiff (PAS) Staining System | Sigma-Aldrich | 395B-1KT | |
| Hematoxylin | Sigma-Aldrich | H3136 | |
| Xylene | MerckMillipore | 108298 | |
| Poly-Prep Slides | Sigma-Aldrich | P0425-72EA | |
| Mounting Medium | ThermoFisher Scientific | 8030 | |
| Hydrophobic Pen | Abcam | ab2601 | |
| Fluorescent Light Miscroscope | Leica Microsystems | ||
| Image J Analysis Software | Image J |
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Cite This Article
Periodic Acid Schiff or PAS Staining: A Method to Assess the Structural Deformities of Glomerulus. J. Vis. Exp. (Pending Publication), e20568, doi: (2023).