H&E Staining of Paraffin-Embedded Tissue Sections: A Differential Staining Technique to Visualize Liver Tissue Sections Using Combination of Hematoxylin and Eosin Dyes

Published: April 30, 2023

Abstract

Source: Ellis, J. L et al., Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish. J. Vis. Exp. (2017).

In this video, we describe a technique of hematoxylin and eosin staining of paraffin-embedded tissue sections to study their histology. The procedure includes deparaffinization of the tissue followed by staining the cells in the tissue. Upon staining, the nucleus appears distinctly blue, and the proteins in the cytoplasm display pink coloration.

Protocol

1. Preparation of staining solutions Filter Harris hematoxylin stock solution to remove any solids that have precipitated. Fold a coffee filter in half so that the filter creates a cone. Open the side panel so that the liquid runs through the filter, allowing any debris to be caught. Place the filter inside a funnel and the funnel into a glass media bottle. Gradually pour the stock hematoxylin through the filter. CAUTION: Hematoxylin is hazardous in…

Disclosures

The authors have nothing to disclose.

Materials

95% ethanol (EtOH) Decon Labs, Inc. 2801 Flammable
Charged slides Fisher Scientific 12-550-16
Dissecting microscope Leica Biosystems Leica Mz 95
Eosin-Phloxine stain set Newcomer Supply 1082A
Ethyl alcohol Sigma-Aldrich E7023 Flammable
Harris hematoxylin Poly Scientific R&D Corp. s212 Irritant
Hydrochloric acid (HCl) Fisher Scientific A144 Irritant
Isopropanol Newcomer Supply 12094E Flammable
Xylenes Fisher Scientific X3S-4 Irritant

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Cite This Article
H&E Staining of Paraffin-Embedded Tissue Sections: A Differential Staining Technique to Visualize Liver Tissue Sections Using Combination of Hematoxylin and Eosin Dyes. J. Vis. Exp. (Pending Publication), e21036, doi: (2023).

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