Establishment of Rice Plant Leaf Infection through Fungal Inoculation via Wounding
Published: October 31, 2023
Abstract
Source: Zhang, M. et al., The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea. J. Vis. Exp. (2018)
This video demonstrates the establishment of rice plant leaf infection through wounding inoculation with Magnaporthe grisea mycelium. The fungal spore-derived germ tube penetrates the epidermal layer, resulting in tissue infection and lesions on the leaves. This method aids pathotype screening and improves plant resistance understanding for molecular breeding.
Protocol
1. Fungal culture for M.grisea Prepare the oatmeal tomato agar (OTA) culture medium for fungal strains. Weigh 30 – 50 g of oatmeal, add this to 800 mL of distilled/deionized water (ddH2O), and boil the mixture for 30 min in the electric pot. Filter the boiled oatmeal juice into the beaker through a piece of gauze. Add 150 mL of tomato juice and 20 g of agar to the filtrate in the beaker and add ddH2O up to 1,000 mL. …
Disclosures
The authors have nothing to disclose.
Materials
| Agar | AOBOX Biotechnology (China) | 01-023 | |
| Filter paper | GE Healthcare brand(Sweden) | 10311387 | |
| Tween-20 | Coolaber (China) | CT11551-100ml | |
| Culture dish | Thermofisher(Amercia) | 150326 | |
| Vacuum pump | Leybold | D25B | |
| Dissection needle | FST | 26000-35 | |
| Incubator | MEMMERT | PYX313 |
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Cite This Article
Establishment of Rice Plant Leaf Infection through Fungal Inoculation via Wounding. J. Vis. Exp. (Pending Publication), e21755, doi: (2023).