A Technique to Detect Extrapulmonary Tuberculosis via Superparamagnetic Nanoprobes

Published: January 31, 2024

Abstract

Source: Lee, C., et al. Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection. J. Vis. Exp. (2020).

This video demonstrates a technique for the detection of extrapulmonary tuberculosis. Superparamagnetic iron oxide (SPIO) nanoprobes, conjugated to Mycobacterium-specific antibodies, are injected into a mouse previously infected with Mycobacterium bovis BCG, labeling the granuloma and providing a better imaging contrast for its detection.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. SPIO-MtbsAb synthesis Synthesize SPIO-conjugated 2,2'-(ethylenedioxy)bisethylamine (EDBE) using previously reported methods. Synthesize SPIO-EDBE-succinic anhydride (SA). Stir an alkaline solution (5 M NaOH; 10 mL)) of SPIO-EDBE and SA (1 g; 10 µmol) at room temperature for 24 h. <l…

Disclosures

The authors have nothing to disclose.

Materials

(benzotriazol-1-yloxy) tripyrrolidinophosphonium hexafluorophosphate Sigma-Aldrich
1-hydroxybenzotriazole Sigma-Aldrich
epichlorohydrin, 2,2'-(ethylenedioxy)bis(ethylamine) Sigma-Aldrich
Human monocytic THP-1
M. bovis BCG Pasteur Mérieux Connaught strain; ImmuCyst Aventis
MRI GE medical Systems 3.0-T, Signa
NMR relaxometer Bruker NMS-120 Minispec
SPECTRUM molecular porous membrane tubing, 12,000 -14,000 MW cut off Spectrum Laboratories Inc
TB surface antibody- Polyclonal Antibody to Mtb Acris Antibodies GmbH BP2027
transmission electron microscope JEOL JEM-2000 EX II

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Cite This Article
A Technique to Detect Extrapulmonary Tuberculosis via Superparamagnetic Nanoprobes. J. Vis. Exp. (Pending Publication), e21895, doi: (2024).

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