Materials
Name | Company | Catalog Number | Comments |
SH-SY5Y cell line | ATCC | CRL-2266 | |
Dulbecco’s Modified Eagles Medium F-12 HAM | Sigma-Aldrich | 51448C | |
Fetal bovine serum | GIBCO, by Life Technologies | 16000-044 | |
MCF7 cell line | ATCC | HTB-22 | |
RPMI-1640 medium | Sigma-Aldrich | R 0883 | |
Apparently normal human fetal fibroblast cell line (NFF) | Coriell Institute for Medical Research | AG04431B | |
Dulbecco’s Modified Eagle’s Medium, High Glucose medium | Thermo Fisher Scientific, Inc. | SH30022.01 | |
Tamoxifen citrate salt | Sigma-Aldrich | T9262 | |
35 mm glass bottom culture dishes | MatTek Corp. | P35G-014-C | |
Leica DMI6000 B inverted microscope | Leica Microsystems | N/A | |
H–chst 33342 dye | Molecular Probes, Life Technologies | H3570 | |
Leica DM IRB inverted fluorescence microscope | Leica Microsystems | N/A | |
Annexin V AlexaFluor-488 | Invitrogen | A13201 | |
Trypan Blue solution | Sigma-Aldrich | T8154-20ML | |
Haemocytometer | Fisher Scientific | 267110 | |
WST-1 reagent | Roche Group | 11644807001 | |
Wallac Victor3 1420 Multilabel Counter | PerkinElmer, Inc. | 1420-011 | |
Tetramethylrhodamine methyl ester (TMRM) | GIBCO, by Life Technologies | T-668 | |
Glass tissue grinder | Fisher Scientific | K8885300-0002 | |
BioRad protein assay | Bio-Rad | 500-0001Bottom of Form | |
Amplex Red | Invitrogen | A12222 | |
Horseradish peroxidase (HRP) | Sigma-Aldrich | P8125 | |
SpectraMax Gemini XPS | Molecular Devices | 3126666 | |
Anti-LC3 antibody raised in rabbit | Novus Biologicals | NB100-2220 | |
Anti-mouse HRP-conjugated secondary antibody | Abcam | ab6728 | |
Anti-rabbit HRP-conjugated secondary antibody | Abcam | ab6802 | |
Chemiluminescence peroxidase substrate | Sigma-Aldrich | CPS160 | |
Monodansylcadaverine | Sigma-Aldrich | 30432 |
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