复透皮电化学传感的空心微针为基础的传感器
Summary
本文详细介绍了建设一个复用微针为基础的传感器。该设备正在研制多种分析快速和选择性地在现场采样和电化学分析。我们设想，临床医学和生物医学研究，这些微针为基础的传感器使用。
Abstract
一种微创复为有关生物分子进行快速分析监测系统的发展，可以提供个人患有慢性疾病的简便评估其直接的生理状态。此外，它可以作为一个复杂的，多因素的医疗条件的分析研究工具。为了实现这样一个多分析传感器，它必须是微创，组织间液的采样必须出现无疼痛或伤害用户，并分析必须迅速以及选择性。
最初发达，无痛苦的给药微针已被用于提供疫苗和药物制剂（如胰岛素）通过皮肤1-2由于这些设备访问的间隙，用微电极集成的微针可以作为透皮电化学传感器。选择性检测葡萄糖，谷氨酸，乳酸，Hydrogen过氧化氢 和抗坏血酸已被证明使用碳纤维，修饰碳糊，铂金涂层的聚合物微针作为换能元件的集成微针电极的装置。3-7,8
这个微针的传感器技术，使一个新的和先进的分析方法，在原位，同时检测多种分析物。复用提供了复杂的微环境监测，否则很难在一个快速，微创的方式来描述的可能性。例如，这项技术可以被利用外同步监测，血糖，乳酸和pH值，9是重要的代谢疾病状态7,10-14指标（如癌症扩散）和运动诱发性酸中毒15。
Protocol
1。微针的制备使用三维造型软件SolidWorks（Dassault Systemes的SA，韦利济，法国），设计一个金字塔形的空心微针阵列（ 图1）。3-5 为支持使用魔法的RP 13软件（Materialise的内华达州，比利时鲁汶）微针阵列结构设计。支撑结构，使树脂从设备在制造过程中流失，并提供了一个建立基地的微针。一个例子支持结构如图1所示。 链接的支持和微?…
Discussion
基于这种微针传感器的设计的多个方面被认为是前设备制造。为了使用这种传感器，实时检测传感器的响应时间必须是低的，在这个协议中，每个测试传感器具有响应时间低于十五秒。其选择性体内环境，其中包含可以干扰与电极反应的电化学生物分子内也选择在本协议中使用的浆料。除了粘贴组成，经营潜力的选择，以尽量减少干扰电活性的影响。微针阵列的制造成功，需要选择一个合?…
Disclosures
The authors have nothing to disclose.
Acknowledgements
桑迪亚是multiprogram的实验室桑迪亚公司是洛克希德 – 马丁公司，美国能源部下属的国家核安全管理局根据合同DE-AC04-94AL85000经营。作者们承认，资金由美国桑迪亚国家实验室的实验室指导研究和开发（LDRD）计划。
Materials
| Name of the reagent | Company | Catalogue number |
| Flat flexible cable | Molex | 3302/10SF |
| 0.003″ Side sided tape | Melinex | |
| 0.004″ Double sided tape | Melinex | |
| Lactate oxidase | Sigma | L0638 |
| Glucose oxidase | Sigma | G7141 |
| Rhodium on carbon | Sigma | 206164 |
| Graphite powder | Sigma | 385031000 |
| poly(ethylenimine) | Acros | 178570010 |
| Mineral oil | Sigma | M5904 |
| Glucose | Sigma | G8270 |
| Lactate | Sigma | L1750 |
| Fast Blue RR salt | Sigma | F0500 |
| e-Shell 300 | EnvisionTEC | |
| e-Shell 200 | EnvisionTEC | |
| Ag/AgCl reference electrode | Basi | MF-2052 |
| Pt wire | Basi | |
| PGSTAT12 AutolabPotentiostat | EcoChemie | |
| Perfactory RP | EnvisionTEC | |
| Ottoflash Postcuring system | EnvisionTEC | |
| Phosphoric acid | Fisher | A366-4 |
| 60W Model 6.75 CO2 raster/vector laser system | Universal Laser Systems | PLS6.75 |
| CorelDraw | Corel | |
| Solidworks | Dassault Systemes | 2009 |
| Magics RP13 | Materialise |
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Tags
Microneedle-based SensorTransdermal Electrochemical SensingMinimally Invasive Monitoring SystemBiologically-relevant MoleculesMultiplexed SensorInterstitial Fluid SamplingRapid AnalysisSelective DetectionMicroneedle-electrode DevicesTransducing ElementsSimultaneous DetectionMultiple AnalytesMicroenvironments
Cite This Article
Miller, P. R., Skoog, S. A., Edwards, T. L., Wheeler, D. R., Xiao, X., Brozik, S. M., Polsky, R., Narayan, R. J. Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing. J. Vis. Exp. (64), e4067, doi:10.3791/4067 (2012).