Materials
| Name | Company | Catalog Number | Comments |
| Feeding Chambers | |||
| Empty Drosophila vial | Genesee Scientific | #32-116 | |
| Absorbent cotton | Any standard product can be used. | ||
| Filter paper | Fisher | #09-802-1B | |
| Paraquat exposure | |||
| Sucrose | Sigma-Aldrich | #S0389 | |
| Paraquat (methyl viologen dichloride hydrate) | Sigma-Aldrich | #856177 | Toxic. Handle with Care and within MSDS guidelines |
| L-NAME (N-Nitro-L-arginine methyl ester hydrochloride) | Sigma-Aldrich | #N5751 | |
| D-NAME (N-Nitro-D-arginine methyl ester hydrochloride) | Sigma-Aldrich | #N4770 | |
| Dissecting Adult Fly Brains and Incubation | |||
| Grace's Insect Medium | Invitrogen | #11605-094 | Grace's Medium may also be substituted for Sf-900 II SFM or Sf-900 III SFM Medium for higher lot-to-lot consistency. |
| 96-well plate | Corning | #3596 | Any standard product can be used. |
| Microscope slide | Any standard product can be used. | ||
| Surgical blade | Feather | #0197 | Any standard product can be used. |
| Medical Tweezers 5 | Dumont | EMS #72877-D | Any standard product can be used. |
| 10x PBS buffer | OmniPur | #6508 | |
| Detection of Nitrite Levels | |||
| Modified Griess reagent | Sigma-Aldrich | #4410 | |
| Sodium nitrite | Sigma-Aldrich | #S2252 or #237213 | |
| Protein Assay Kit | Bio-Rad | 500-0001 | |
| Cuvettes | Any standard product can be used. | ||
| Nitrate reductase | optional | ||
| NADPH | optional | ||
| Western Blot Analysis | |||
| RIPA lysis buffer | Amresco | 97063-270 | |
| dithiothreitol (DTT)- Reducing agent | Novex | B0009 | |
| Protease inhibitor cocktail | Amresco | 97063-082 | |
| 4x LDS sample buffer | Novex | B0007 | |
| Precast 4-12% Bis-Tris minigel | Novex | BG04125BOX | |
| MOPS SDS running buffer | Novex | B0001 | |
| Antioxidant | NuPage | NP0005 | |
| iBlot Transfer Stacks (Nitrocellulose) | Invitrogen | IB301002 | |
| Western blot detection kit | Invitrogen | IB711002 | |
| Mouse α-nNOS | BD Biosciences | 611853 | |
| Mouse α-syntaxin | DSHB | 8C3 | |
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