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Journal
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Immunology and Infection
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监测改变膜极性,膜完整性和细胞内离子浓度<em>肺炎链球菌</em>使用荧光染料
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in
Streptococcus pneumoniae
Using Fluorescent Dyes
Please note that all translations are automatically generated.
Click here for the English version.
监测改变膜极性,膜完整性和细胞内离子浓度<em>肺炎链球菌</em>使用荧光染料
DOI:
10.3791/51008-v
•
11:17 min
•
February 17, 2014
•
Emily A. Clementi*
1
,
Laura R. Marks*
1
,
Hazeline Roche-Håkansson
,
Anders P. Håkansson
2,3
1
Department of Microbiology and Immunology
,
University at Buffalo, State University of New York
,
2
Witebsky Center for Microbial Pathogenesis and Immunology
,
University at Buffalo, State University of New York
,
3
New York State Center of Excellence in Bioinformatics and Life Sciences
,
University at Buffalo, State University of New York
Chapters
00:05
Title
01:15
Detecting Membrane Depolarization and Rupture
02:58
Detecting Changes in Intracellular Ca
2+
or K
+
Concentrations
05:31
Detecting Changes in Intracellular pH
08:56
Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations
10:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
不像见过的真核生物中,有研究贫乏,在细菌细节膜去极化和离子浓度的变化,主要是由于它们的小尺寸使得难以测量的常规方法。在这里,我们监测的显著革兰氏阳性病原体
肺炎链球菌
利用荧光技术这样的事件细节的协议。
Tags
Membrane Polarity
Membrane Integrity
Intracellular Ion Concentrations
Fluorescent Dyes
Streptococcus Pneumoniae
Membrane Depolarization
Ion Fluxes
Fluorescent Indicators
DiBAC4(3)
Propidium Iodide
Fura-2
PBFI
BCECF
Fluorescence Detection
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