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Immunology and Infection

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JoVE Journal Immunology and Infection
Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation
 

Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation

Article DOI: 10.3791/55949-v • 12:48 min • August 21st, 2017
August 21st, 2017 •
1, 1, 1, 1
1Laboratory of Physiological Chemistry, Faculty of Pharmacy, Takasaki University of Health and Welfare

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Summary
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The method described here is a new vesicle isolation protocol, which allows for the purification of the cellular compartments where exogenous antigens are processed by endoplasmic reticulum-associated degradation in cross-presentation.

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Keywords: Membrane Compartment Endoplasmic Reticulum-associated Degradation (ERAD) Cross-presentation Exogenous Antigen Dendritic Cells Biotinylated Ovalbumin (bOVA) Vesicle Isolation Microsome Ubiquitination
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