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外层膜蛋白的分离线粒体的流式细胞仪免疫检测

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JoVE Journal Biology

Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria

Please note that all translations are automatically generated. Click here for the English version.

外层膜蛋白的分离线粒体的流式细胞仪免疫检测

DOI:

10.3791/51887-v

•

11:53 min

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September 18, 2014

•

Sarah Pickles, Nathalie Arbour, Christine Vande Velde

¹Department of Biochemistry,Université de Montréal, CRCHUM, ²Department of Neurosciences,Université de Montréal, CRCHUM

Chapters

00:05Title
01:54Isolation of Spinal Cord Mitochondria
05:34Immunolabeling of Isolated Mitochondria for Flow Cytometry
08:06Assaying Mitochondrial Transmembrane Potential and Superoxide Production by Flow Cytometry
09:30Acquisition of Immunolabeled Isolated Mitochondria by Flow Cytometry
10:21Results: Flow Cytometry Analysis of Isolated Mitochondria
11:34Conclusion

Summary

Automatic Translation

这里描述的是这样一种方法来检测并通过免疫标记线粒体分离从啮齿动物的组织和通过流式细胞仪分析的量化线粒体外膜蛋白质。该方法可以被扩展以评估线粒体亚群的功能方面。

Tags

Mitochondrial Outer Membrane Proteins Flow Cytometry Immunolabeling Mitochondrial Isolation Spinal Cord Mitochondrial Purity High-throughput Analysis Mitochondrial Subpopulations Fluorescent Indicator Dyes

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