Summary

Taze izole edilmiş insan Villöz sitotrofoblastlar ilgili siRNA transfeksiyonu ve EMSA analizi

Published: September 20, 2016
doi:

Summary

Bu protokol, etkili bir şekilde, taze izole edilmiş beşeri villöz sitotrofoblastlar siRNA transfekte gözeneklendirilmesinin kullanılarak bu hücrelerde DNA-protein kompleksleri tanımlanması için bir yöntem tarif eder. Transfekte edilen hücreler, Western Blot ile izlenebilir ve EMSA 4-günlük kültür döneminde analiz eder.

Abstract

Human primary villous cytotrophoblasts are a very useful source of primary cells to study placental functions and regulatory mechanisms, and to comprehend diseases related to pregnancy. In this protocol, human primary villous cytotrophoblasts freshly isolated from placentas through a standard DNase/trypsin protocol are microporated with small interfering RNA (siRNA). This approach provided greater efficiency for siRNA transfection when compared to a lipofection-based method. Transfected cells can subsequently be analyzed by standard Western blot within a time frame of 3-4 days post-transfection. In addition, using cultured primary villous cytotrophoblasts, Electrophoretic Mobility Shift Assay (EMSA) analysis was optimized and performed on extracts from days 1 to 4. The use of these cultured primary cells and the protocol described allow for an evaluation of the implication of specific genes and transcription factors in the process of villous cytotrophoblast differentiation into a syncytiotrophoblast-like cell layer. However, the limited time span allowable in culture precludes the use of methods requiring more time, such as generation of a stable cell population. Therefore testing of this cell population requires highly optimized gene transfer protocols.

Introduction

Human placental dysfunction is associated with the development of several pregnancy-associated diseases like preeclampsia and intrauterine growth restriction 1. An important cell constituent of the placenta is the trophoblasts, which can be classified as either extravillous or villous cytotrophoblasts. Upon fusion, villous cytotrophoblasts further differentiate into the syncytiotrophoblast layer, a multinuclear cell structure with an important role in feto-maternal exchange and hormone production 2. Human primary villous cytotrophoblasts and their differentiated counterparts represent important biological samples and allow researchers to study a number of placenta-related processes, such as cell fusion, in culture. Furthermore, substantial efforts are ongoing to identify markers that will facilitate appropriate management and improve preventive therapies specific to pregnancy-related diseases. Laboratories routinely isolate primary human villous cytotrophoblasts from fresh placentas, using a standard isolation procedure based on trypsin digestion of placenta villi 3. As cultured cytotrophoblasts lose their capacity to proliferate and quickly differentiate in a syncytiotrophoblast-like layer upon culture 4, very efficient transfection methods and optimized analysis approaches are needed. Previous studies have determined optimal conditions of transfection of these primary cytotrophoblasts 5. Herein, a different method of siRNA transfection, which has been previously tested in this cell type 6, is presented. In comparison to a lipofection-based approach, this microporation method improves transfection, as assessed by the extent of silencing of specific genes.

Promoter and gene expression studies also provide a better understanding of placental function. Although more difficult to use owing to the short time frame for which primary villous cytotrophoblasts can be cultured, promoter analyses using standard protocols can nonetheless be addressed, as previously published 7. Electrophoretic Mobility Shift Assay (EMSA) is one of these commonly used in vitro methods, allowing for fast and easy monitoring of DNA-protein interactions. Nuclear extracts from these primary trophoblasts were used to test a region of the Syncytin-2 promoter for specific interactions. Results revealed that bound factors could be detected at different time points of culture and in a specific and reproducible manner.

Data presented in this protocol confirm that our transfection approach and the EMSA protocol can be used for isolated primary villous cytotrophoblasts and will be of great use to study the diverse functions of villous cytotrophoblasts in normal or pathological conditions.

Protocol

Uqam etik kurul Centre Hospitalier Universitaire de Montréal St-Luc Hastanesi (Montréal, Kanada) etik komitenin kurallarına uygun olan bu protokolleri onayladı. Katılımcılar bilgilendirilmiş onam formunu imzaladı. 1. Orta Hazırlama ve İlköğretim Villöz sitotrofoblastlar İzolasyonu 25 mM HEPES,% 10 fetal sığır serumu (FBS) ve% 1 penisilin / streptomisin içeren Dulbecco Modifiye Kartal Ortamı (DMEM) ilave etmek suretiyle, insan primer villöz sitotrofoblastlar için kültür o…

Representative Results

terimi, gebelik taze plasenta Protokol bölümde sunulan deneyler grubunu yapmak için insan primer villöz sitotrofoblastlar izole etmek için kullanılmıştır. Bunların izolasyonundan sonra, ilk sitokeratin-7 işaretleyici (Şekil 1) kullanılarak sitotrofoblastlar saflığını analiz edilmiştir. Hücre preparatları, böylece, bir monoklon anti-sitokeratin-7 antikoru kullanılarak boyandı. 1 sitometri, standart akış sitometrisi ile analiz prim…

Discussion

insan plasental fonksiyon ve geliştirme ile ilgili çalışmalar büyük ölçüde çeşitli plasental hücre popülasyonlarının izolasyonu optimize hedefleyen protokoller tarafından geliştirilmiştir. En iyi çalışılmış plasental hücre popülasyonunun biri büyük ölçüde verimli ve güvenilir bir izolasyon izin optimize protokolleri yararlandı çalışma olan villöz sitotrofoblastlar kalır. Bu ayrıca, transfeksiyon ve promotör çalışmaları gibi deneyler, bir dizi izin vermektedir. Daha önce tarif…

Disclosures

The authors have nothing to disclose.

Acknowledgements

Bu çalışma Kanada Ulusal Bilimler ve Mühendislik Araştırma Konseyi (NSERC) (# 298527) (BB) bir hibe ile desteklenmiştir. BT kurumsal FARE burs tarafından desteklenmiştir. AV NSERC Graham Bell Ph.D. tarafından desteklenen burs. BB İnsan Retrovirology bir Kanada Araştırma Başkanı (Tier 2) düzenledi. metnini revize yardım için Beatrix Beisner teşekkürler.

Materials

HBSS without  Ca2+, Mg2+  Sigma #H2387
HBSS (10X)  Sigma #14060-057
DMEM High Glucose without Hepes Gibco  #12100-061
Hepes (1 M) Gibco #15630-080
Penicillin-Streptomycin-Neomycin (100X) Gibco  #15640-055 
Amphotericin B  Sigma #A2411
CaCl2 Sigma  #C4901
MgSO4.7H2 Sigma  #M
Fetal bovine serum Gibco #16170-078 
Percoll  Sigma  #P-1644  For density gradient
Syncytin-2 siRNA Ambion Life technologies #AM16708
Scrambled siRNA Qiagen # SI03650318
DNase I   Sigma-Aldrich #D5025
Trypsine, type I  Sigma  #T8003
DharmaFECT Lipotransfection  reagents  GEhealthcare # T-2001-01
Trypsin/EDTA  Life technologies #25300-062
Protease Inhibitor Cocktail Roche Diagnostic #11873580001
Pierce BCA Protein Assay Kit Thermo Scientific #23225
BSA Sigma #A7906
TWEEN 20 Sigma #P9416 
Anti-rabbit IgG, HRP-linked antibody  Cell Signaling #7074
BM Chemiluminescence Western Blotting Substrate (POD) Roche Diagnostic #11500708001
DPBS  Life technologies #14287-080
T4 Polynucleotide Kinase NEB #M0201S
ATP, [γ-32P] Perkin Elmer  #BLU002A100UC 
Acrylmide Sigma  #A9099
TEMED Life technologies #17919
Ammonium Persulfate Sigma  #A3678
Anti-human cytokeratin-7  antibody clone LP5K, FITC conjugated Millipore,  CBL194F Dilution1:200 
 FcR blocking reagent   Miltenyi Biotec  130- 059-901  Dilution 1:10
Flow Cytometer BD Acuri system  Becton Dickinson
Microporator MP-100 apparatus  Digital Bio
Resuspension Buffer R (Neon Transfection System 100 µL Kit)  Life technologies MPK10096
PVDF membrane  Millipore IPVH00010  Activate with methanol
Anti-human GAPDH antibody  Santa Cruz Biotechnology  sc-137179  1:500
HorseRadish Peroxidase (HRP)-conjugated goat anti-rabbit antibody or anti-mouse antibody  Cell Signalling  #7074   1:10,000
HorseRadish Peroxidase (HRP)-conjugated goat anti-mouse antibody  Cell Signalling  #7076   1:10,000
 NE-PER Nuclear and Cytoplasmic Extraction Reagent Thermo Scientific #78833
G-25 column  GE Healthcare #27-5325-01
Chemiluminsescence and fluorescence imaging device Montréal Biotech Fusion FX5
 4 % native gel Home made
PBS Home made 1X
Personal Molecular Imager (PMI) System BioRad

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Cite This Article
Lokossou, A. G., Toufaily, C., Vargas, A., Barbeau, B. siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts. J. Vis. Exp. (115), e53995, doi:10.3791/53995 (2016).

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