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用肽微阵列分析组蛋白抗体特异性

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Analysis of Histone Antibody Specificity with Peptide Microarrays

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用肽微阵列分析组蛋白抗体特异性

DOI:

10.3791/55912-v

•

09:47 min

•

August 01, 2017

•

Evan M. Cornett, Bradley M. Dickson, Scott B. Rothbart

¹Center for Epigenetics,Van Andel Research Institute

Chapters

00:05Title
01:09Array Slide and Source Plate Layout Design
02:13Microarray Fabrication and Slide Partitioning
04:46Histone Post-translational Modification (PTM) Antibody Hybridization
06:14Data Analysis and Quantification
08:03Results: Analysis of Histone PTM Antibody Specificity with ArrayNinja
08:55Conclusion

Summary

Automatic Translation

该手稿描述了将肽微阵列技术应用于识别组蛋白及其翻译后修饰的抗体的特异性分析的方法。

Tags

Histone Antibody Peptide Microarray Post-translational Modification Chromatin Biochemistry Antibody Specificity Array Ninja Microarray Design Microarray Printing Feature Identification Source Plate Map Protein Microarray Printing Buffer Fluorescein-labeled Biotin Microarray Printing Software

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