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Biochemistry
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用肽微阵列分析组蛋白抗体特异性
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Analysis of Histone Antibody Specificity with Peptide Microarrays
Please note that all translations are automatically generated.
Click here for the English version.
用肽微阵列分析组蛋白抗体特异性
DOI:
10.3791/55912-v
•
09:47 min
•
August 01, 2017
•
Evan M. Cornett
,
Bradley M. Dickson
,
Scott B. Rothbart
1
Center for Epigenetics
,
Van Andel Research Institute
Chapters
00:05
Title
01:09
Array Slide and Source Plate Layout Design
02:13
Microarray Fabrication and Slide Partitioning
04:46
Histone Post-translational Modification (PTM) Antibody Hybridization
06:14
Data Analysis and Quantification
08:03
Results: Analysis of Histone PTM Antibody Specificity with ArrayNinja
08:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该手稿描述了将肽微阵列技术应用于识别组蛋白及其翻译后修饰的抗体的特异性分析的方法。
Tags
Histone Antibody
Peptide Microarray
Post-translational Modification
Chromatin Biochemistry
Antibody Specificity
Array Ninja
Microarray Design
Microarray Printing
Feature Identification
Source Plate Map
Protein Microarray Printing Buffer
Fluorescein-labeled Biotin
Microarray Printing Software
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