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Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
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Biochemistry
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JoVE Journal Biochemistry
Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

DOI:
10.3791/59886-v

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13:22 min

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October 23, 2019

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,
1Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute,Arizona State University, 2Department of Pediatrics, and Biochemistry/Molecular Biology, College of Medicine,Pennsylvania State University

Chapters

  • 00:05Title
  • 01:01DNA Preparation
  • 04:00Aminosilane Slide Coating and Array Sample Preparation
  • 05:29Microarray Printing and Measurement of DNA levels on NAPPA Slides
  • 06:24Expression of NAPPA Arrays
  • 07:29Detection of Proteins on NAPPA Arrays
  • 08:23Tyrosine Kinase Inhibitor Screening on NAPPA Arrays
  • 10:41Results: Protein Expression
  • 12:25Conclusion

Summary

Automatic Translation

Automatic Translation

A detailed protocol for the generation of self-assembled human protein microarrays for the screening of kinase inhibitors is presented.

Tags

Keywords: NAPPAProtein MicroarrayKinase Inhibitor ScreeningHuman ProteinProtein ActivityProtein FunctionHigh-throughputBiomarker DiscoveryProtein-protein InteractionsSubstrate IdentificationDrug ScreeningQuality ControlBacterial GrowthHigh-throughput Mini-prepTerrific Broth MediumAmpicillin96-pin DeviceDeep Well PlateShakerBacterial LysisAnion Exchange Resin Plates

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