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낮은 풍부 배아 샘플 chromatin Immunoprecipitation (칩) 프로토콜
JoVE Journal
Genetics
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JoVE Journal Genetics
Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples
DOI:

12:47 min

August 29, 2017

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Chapters

  • 00:05Title
  • 00:57Preparation of Eggs and Microdissection of Chicken Spinal Neural Tube (SNT)
  • 04:12Crosslinking Proteins to DNA
  • 05:01Lysis, Sonication, and Immunoprecipitation
  • 06:33Washing, Elution, and Reversal of Crosslinking
  • 08:57Cellular Protein and RNA Digestion
  • 10:06Results: Evaluation of the ChIP Protocol by ChIP-seq Experiments
  • 11:48Conclusion

Summary

Automatic Translation

여기, 우리는 chromatin immunoprecipitation (칩) 및 칩 seq 라이브러리 준비 프로토콜 낮은 풍부한 닭 배아 샘플에서 글로벌 epigenomic 프로 파일 생성을 설명 합니다.

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