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At studere hypothalamus Insulin Signal til perifere glukose Intolerance med en løbende Drug Infusion System ind i musen hjernen
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Medicine
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JoVE Journal Medicine
Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain

At studere hypothalamus Insulin Signal til perifere glukose Intolerance med en løbende Drug Infusion System ind i musen hjernen

DOI:
10.3791/56410-v

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08:32 min

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January 04, 2018

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,
1The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University and National Health Research, 2Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology,Taipei Medical University, 3TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology,Taipei Medical University

Chapters

  • 00:05Title
  • 01:21Preparation of Micro-osmotic Pump Infusion Systems
  • 02:41Intracerebral Ventricular Surgery – Implantation of the Micro-osmotic Pump
  • 04:15Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)
  • 06:04Results: Glucose Metabolism and Insulin Signal Activation
  • 07:27Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol undersøgelser af chemokine (C-C motiv) ligand 5 (CCL5) i hypothalamus rolle ved at levere en antagonist, MetCCL5, ind i musen hjernen ved hjælp af en mikro-osmotisk pumpe hjernen infusion system. Denne forbigående hæmning af CCL5 aktivitet afbrudt hypothalamus insulin signalering, fører til glukose intolerance og perifere systemisk insulinfølsomhed.

Tags

Hypothalamic Insulin SignalPeripheral Glucose IntoleranceC-C-L-5 SignalingMet-C-C-L-5Micro-osmotic PumpArtificial Cerebrospinal FluidBrain Infusion KitStereotactic ApparatusAnesthetized MouseBrain Map

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