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人心脏组织对细胞外基质自组装水凝胶的处理体外体内应用
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
DOI:

08:41 min

December 04, 2017

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Chapters

  • 00:05Title
  • 00:43Sectioning Human Myocardium into Slices
  • 02:27Decellularization of Human Myocardium Slices
  • 04:20Processing the Decellularized cECM to a Powder
  • 05:50Enzyme-based Homogenization of cECM Micro-particles
  • 06:56Results: Effective Removal of Cellular Material Enhances Analysis
  • 08:10Conclusion

Summary

Automatic Translation

该协议描述了人类心肌的完整细胞, 同时保留了其胞外基质成分。细胞外基质的进一步处理导致微粒和细胞自组装水凝胶的生产。

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