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Journal
/
Medicine
/
念珠菌光滑
全基因组测序检测抗真菌耐药性标志物
JoVE Journal
Medicine
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JoVE Journal
Medicine
Whole Genome Sequencing of
Candida glabrata
for Detection of Markers of Antifungal Drug Resistance
Please note that all translations are automatically generated.
Click here for the English version.
念珠菌光滑
全基因组测序检测抗真菌耐药性标志物
DOI:
10.3791/56714-v
•
08:45 min
•
December 28, 2017
•
Chayanika Biswas*
1
,
Sharon C-A. Chen*
1,2,3
,
Catriona Halliday
2
,
Elena Martinez
,
Rebecca J. Rockett
,
Qinning Wang
,
Verlaine J. Timms
,
Rajat Dhakal
,
Rosemarie Sadsad
,
Karina J. Kennedy
,
Geoffrey Playford
5
,
Deborah J. Marriott
,
Monica A. Slavin
,
Tania C. Sorrell
3
,
Vitali Sintchenko
2,3
1
Centre for Infectious Diseases and Microbiology-Public Health
,
Westmead Hospital
,
2
Centre for Infectious Diseases and Microbiology Laboratory Services
,
ICPMR
,
3
Marie Bashir Institute for Infectious Diseases and Biosecurity
,
The University of Sydney
,
4
Department of Microbiology and Infectious Diseases, Canberra Hospital and Health Services
,
Australian National University Medical School
,
5
Infection Management Services
,
Australian National University Medical School
,
6
Department of Microbiology and Infectious Diseases
,
St. Vincent’s Hospital
,
7
Department of Infectious Diseases
,
Peter MacCallum Cancer Centre
Chapters
00:05
Title
01:11
Tagmentation and PCR Amplification
03:35
PCR Cleanup
05:15
Library Normalization
07:19
Results: Susceptibility Profiles of
Candida glabrata
Isolates Correlates with Presence or Absence of Specific Gene Mutations
08:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这项研究实施了全基因组测序分析的基因突变, 赋予抗真菌药物耐药性的
念珠菌光滑
。对棘、唑和 5-嘧啶的耐
光滑
的菌株进行了测序, 以说明方法。分离株的敏感性分布与基因特异性突变模式的存在或缺失相关。
Tags
Whole Genome Sequencing
Candida Glabrata
Antifungal Drug Resistance
Genomic DNA
Tagmentation
PCR
Indexing
Cross-contamination
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