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念珠菌光滑全基因组测序检测抗真菌耐药性标志物
JoVE Journal
Medicine
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JoVE Journal Medicine
Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
DOI:

08:45 min

December 28, 2017

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Chapters

  • 00:05Title
  • 01:11Tagmentation and PCR Amplification
  • 03:35PCR Cleanup
  • 05:15Library Normalization
  • 07:19Results: Susceptibility Profiles of Candida glabrata Isolates Correlates with Presence or Absence of Specific Gene Mutations
  • 08:11Conclusion

Summary

Automatic Translation

这项研究实施了全基因组测序分析的基因突变, 赋予抗真菌药物耐药性的念珠菌光滑。对棘、唑和 5-嘧啶的耐光滑的菌株进行了测序, 以说明方法。分离株的敏感性分布与基因特异性突变模式的存在或缺失相关。

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