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均匀时间分辨 Förster 共振能量转移法检测胰岛素分泌

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JoVE Journal Medicine

Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion

Please note that all translations are automatically generated. Click here for the English version.

均匀时间分辨 Förster 共振能量转移法检测胰岛素分泌

DOI:

10.3791/57531-v

•

07:30 min

•

May 10, 2018

•

Despoina Aslanoglou, Emily W. George, Zachary Freyberg²

¹Department of Psychiatry,University of Pittsburgh, ²Department of Cell Biology,University of Pittsburgh

Chapters

00:05Title
01:22INS-1E Cells: Maintenance and Plating
03:04Insulin Secretion Assay
04:12HTRF To Measure Insulin Secretion and Data Analysis
05:17Results: Use of HTRF Assay for the Rapid Detection of Insulin Secretion from the Cells
06:43Conclusion

Summary

Automatic Translation

在这里, 我们提出的均匀时间解决烦恼 (HTRF) 作为一种有效的方法来快速检测胰岛素分泌的细胞。

Tags

Insulin Secretion Time-resolved FRET Beta Cells Glucose Stimulation Diabetes Drug Discovery INS-1E Cells Cell Culture Trypsin Cell Counting Viability

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