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均匀时间分辨 Förster 共振能量转移法检测胰岛素分泌
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Medicine
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JoVE Journal
Medicine
Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
Please note that all translations are automatically generated.
Click here for the English version.
均匀时间分辨 Förster 共振能量转移法检测胰岛素分泌
DOI:
10.3791/57531-v
•
07:30 min
•
May 10, 2018
•
Despoina Aslanoglou
,
Emily W. George
,
Zachary Freyberg
2
1
Department of Psychiatry
,
University of Pittsburgh
,
2
Department of Cell Biology
,
University of Pittsburgh
Chapters
00:05
Title
01:22
INS-1E Cells: Maintenance and Plating
03:04
Insulin Secretion Assay
04:12
HTRF To Measure Insulin Secretion and Data Analysis
05:17
Results: Use of HTRF Assay for the Rapid Detection of Insulin Secretion from the Cells
06:43
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出的均匀时间解决烦恼 (HTRF) 作为一种有效的方法来快速检测胰岛素分泌的细胞。
Tags
Insulin Secretion
Time-resolved FRET
Beta Cells
Glucose Stimulation
Diabetes
Drug Discovery
INS-1E Cells
Cell Culture
Trypsin
Cell Counting
Viability
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