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通过检测全小鼠胚胎β乳糖酶活性追踪基因表达
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos
DOI:

08:42 min

June 26, 2018

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Chapters

  • 00:04Title
  • 00:49β-galactosidase Histochemistry of Whole Mount Mouse Embryos
  • 02:23Paraffin Embedding of X-gal Stained Embryos
  • 04:37Paraffin Sectioning
  • 05:44Deparaffinization and Counterstaining
  • 06:56Results: X-gal Staining of E9.5 WT and Mt4-mmpLacZ/ Whole-mount Embryos and Tissue Sections
  • 07:52Conclusion

Summary

Automatic Translation

在这里, 我们描述了检测早期全小鼠胚胎β乳糖酶活性的标准协议, 以及石蜡切片和 counterstaining 的方法。这是一个简单和快速的过程, 以监测基因表达在发展期间, 也可以应用于组织切片, 器官或培养细胞。

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