Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Visualizing the Node and Notochordal Plate In…
JoVE Journal
Developmental Biology

This content is Open Access.

JoVE Journal Developmental Biology
Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence
 

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

Article DOI: 10.3791/58321-v • 09:36 min • November 6th, 2018
November 6th, 2018 •
1,2,3, 4, 2,3
1Graduate Program in Pharmacology and Experimental Therapeutics, University of Cologne, 2Department of Dermatology and Venereology, University Hospital of Cologne, 3Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 4Department of General Ecology, Institute for Zoology, Biocenter Cologne, University of Cologne

Chapters

Summary
Automatic Translation

The node and notochordal plate are transient signaling organizers in developing mouse embryos that can be visualized using several techniques. Here, we describe in detail how to perform two of the techniques to study their structure and morphogenesis: 1) scanning electron microscopy (SEM); and 2) whole mount immunofluorescence (WMIF).

Tags

Keywords: Node Notochordal Plate Mouse Embryo Scanning Electron Microscopy (SEM) Whole Mount Immunofluorescence Embryonic Development Dissection Fixation Dehydration Genotyping Reichert's Membrane Yolk Sac
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter