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Chapters
- 00:05Title
- 01:16Reconstitution of Target Ready Vials
- 02:17Reconstitution of Untargeted Vials
- 02:59Coulter Counting
- 03:45Preparing Microbubbles for Injection
- 05:37Injection of Microbubbles into Embryos
- 09:02Results: Ultrasound Imaging of Mouse Embryos with Microbubbles
- 10:18Conclusion
Here, we present a protocol to inject ultrasound microbubble contrast agents into living, isolated late-gestation stage murine embryos. This method enables the study of perfusion parameters and of vascular molecular markers within the embryo using contrast-enhanced high-frequency ultrasound imaging.
