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5-メチルシトシンと開発の 5-ヒドロキシメチルシトシンの免疫組織化学的検出と後マウス網膜
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina
DOI:

07:50 min

August 29, 2018

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Chapters

  • 00:04Title
  • 00:59Tissue Preparation for Immunostaining
  • 03:02Immunostaining with 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) Antibodies
  • 05:02Results: Immunostaining with 5mC and 5hmC to Determine Their Distribution During Retinal Development
  • 06:49Conclusion

Summary

Automatic Translation

このレポートの目的は、エピジェネティックな遺伝子マーカーの堅牢な免疫組織化学的検出のためのプロトコルを記述するためです、5-メチルシトシン (5mC) および 5-ヒドロキシメチルシトシン (5hmC) の開発と後マウス網膜。

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