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Medicine
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非放射性 l-azidohomoalanine 标记法分离原发性小鼠肝细胞对新生蛋白合成的研究
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JoVE Journal
Medicine
Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
Please note that all translations are automatically generated.
Click here for the English version.
非放射性 l-azidohomoalanine 标记法分离原发性小鼠肝细胞对新生蛋白合成的研究
DOI:
10.3791/58323-v
•
08:04 min
•
October 23, 2018
•
Esam S.B. Salem*
1,2
,
Kazutoshi Murakami*
2
,
Toshimasa Takahashi
,
Elise Bernhard
,
Vishnupriya Borra
,
Mridula Bethi
,
Takahisa Nakamura
3,4
1
Department of Pharmacology and Systems Physiology, College of Medicine
,
University of Cincinnati
,
2
Division of Endocrinology
,
Cincinnati Children’s Hospital Medical Center
,
3
Division of Developmental Biology
,
Cincinnati Children’s Hospital Medical Center
,
4
Department of Pediatrics, College of Medicine
,
University of Cincinnati
Chapters
00:04
Title
00:47
Liver Perfusion and Extraction
02:45
Primary Mouse Hepatocyte Isolation
04:07
Polyacrylamide Gel Electrophoresis (PAGE) Analysis
06:06
Results: Representative Nascent Protein Expression Analyses
07:31
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个分离健康和功能的原发性小鼠肝细胞的协议。通过非放射性标记基底检测肝新生蛋白合成的说明, 帮助了解肝脏能量代谢稳态背景下蛋白质合成的机理。
Tags
Primary Mouse Hepatocytes
Nascent Protein Synthesis
L-azidohomoalanine Labeling
Non-radioactive
Liver Perfusion
Collagenase
Density Gradient Separation
Metabolic Disease Research
Obesity
Non-alcoholic Fatty Liver
Type Two Diabetes
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