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非放射性 l-azidohomoalanine 标记法分离原发性小鼠肝细胞对新生蛋白合成的研究
JoVE Journal
Medicine
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JoVE Journal Medicine
Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
DOI:

08:04 min

October 23, 2018

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Chapters

  • 00:04Title
  • 00:47Liver Perfusion and Extraction
  • 02:45Primary Mouse Hepatocyte Isolation
  • 04:07Polyacrylamide Gel Electrophoresis (PAGE) Analysis
  • 06:06Results: Representative Nascent Protein Expression Analyses
  • 07:31Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个分离健康和功能的原发性小鼠肝细胞的协议。通过非放射性标记基底检测肝新生蛋白合成的说明, 帮助了解肝脏能量代谢稳态背景下蛋白质合成的机理。

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